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The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

Received: 8 November 2014     Accepted: 12 November 2014     Published: 14 November 2014
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Abstract

Exposure to ionizing radiation leads to exhaustion of the pool of stem cells, increases differentiated cell stress, and activated reparation and apoptosis processes. Apart from well-documented acute effects, radiotherapy leads to delayed effects manifesting in few years after successful treatment. The aim of our study was the development of a test battery for evaluation of irradiation aftereffects in organisms characterized by different radiosensitivity. We chose C57BL mice as the ideal strain, because according to published data, they are characterized by medium radiosensitivity. At the preliminary stage, we have to assess informative value of some methods for the sublethal irradiation dose of 750 R. For evaluation of the damaging effects of radiation (lymphocyte count and maturity), we used, along with classical criteria, some complex parameters (body weight, serum homeostasis, behavior) characterizing general status of the organism. We also performed histological analysis of the liver, spleen, and pancreas and ranged the injuries by their severity.

Published in American Journal of Life Sciences (Volume 3, Issue 1-2)

This article belongs to the Special Issue Space Flight Factors: From Cell to Body

DOI 10.11648/j.ajls.s.2015030102.12
Page(s) 5-12
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Histological Examination, Complex of Tests, Laser Correlation Spectroscopy, Radiation

References
[1] "O.I. Stepanova, “Alternative method of blood sampling from mouse’s saphenous vein,” Biomedicine, No.2, 2006, pp. 137-139 (in Russian).
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[3] I. Alchinova, E. Arkhipova, A. Cherepov and M. Karganov, “Polysystemic monitoring of nuclear plant stuff and risk group revealing,” In: Risk assessment and management. Zhang Zhiyong ed., 2012, Academy Publish, USA. ISBN: 978-0-9835850-2-2. P. 193-199. http://www.academypublish.org/book/show/title/risk-assessment-and-management.
[4] G.N. Kryzhanovsky, “Some categories of general pathology and biology: health, disease, homeostasis, sanogenesis, adaptation, immunity. New approaches and notions,” Pathophysiology, Vol.11, No.2, 2004, pp.135-138.
[5] I.E. Kovalev, M.Y. Karganov, E.I. Rumyantseva and O.I. Kovaleva, “Laser correlation spectroscopy as an effective method of detection of DNA-containing and other macromolecular complexes in blood serum of patients with diabetes mellitus,” Doklady Biochemistry and Biophysics, Vol. 386, No 1-6, 2002, pp. 281-283.
[6] Piruzyan L.A., Kovalev I.E., Rumyantseva E.I., Kovaleva V.L., Tyumentseva E.S., Balabolkin I.I., Karganov M.Yu., and O.I. Kovaleva, “Laser correlation spectroscopy of macromolecular complexes in blood serum as an effective method of monitoring the progress of bronchial asthma in children,” Doklady Biochemistry and Biophysics, Vol. 395, No 1-6, 2004, pp. 114-117.
[7] Karganov M.Y., Kozhevnikova M.I., Aleschenko A.V., Khlebnikova N.N., Dmitrieva O.S., Alchinova I.B., Dmitrieva G.P., Pelevina I.I., and L.A. Noskin, “Correlation between cytogenic abnormalities in cells and metabolic shifts during spinal deformities in children,” Studies in Health Technology and Informatics, Vol.123, 2006, pp.9-13.
[8] M. Karganov, A. Skalny, I. Alchinova, N. Khlebnikova, A. Grabeklis, E. Lakarova, S. Eisazadeh, “Combined use of laser correlation spectroscopy and ICP-AES, ICP-MS determination of macro- and trace elements in human biosubstrates for intoxication risk assessment,” Trace elements and electrolytes, Vol.28, No.2, 2011, pp.124-127
[9] I. Alchinova, E. Arkhipova, D. Sidnev, A. Sanadze, S. Dedaev, and M. Karganov, “Comparative Analysis of the Informative Value of Radioimmunoassay and Laser Correlation Spectroscopy in Myasthenia Gravis,” ISRN Immunology, 2014, Article ID 718393, 5 pages, 2014. doi:10.1155/2014/718393, http://www.hindawi.com/journals/isrn.immunology/2014/718393/
[10] Karganov M., Alchinova I., Arkhipova E., and A.V. Skalny, “Laser Correlation Spectroscopy: Nutritional, Ecological and Toxic Aspects,” In: “Biophysics”. A.N. Misra ed.- InTech, 2012, -ISBN 978-953-51-0376-9. pp.1-16. DOI : 10.5772/35254 http://www.intechopen.com/articles/show/title/laser-correlation-spectroscopy-nutritional-ecological-and-toxic-aspects
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[15] Chu D., “Laser light scattering,” N.Y.: Acad. Press., 1974, 317 p "
Cite This Article
  • APA Style

    Irina Alchinova, Elena Arkhipova, Yulia Medvedeva, Anton Cherepov, Alexander Antipov, et al. (2014). The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals. American Journal of Life Sciences, 3(1-2), 5-12. https://doi.org/10.11648/j.ajls.s.2015030102.12

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    ACS Style

    Irina Alchinova; Elena Arkhipova; Yulia Medvedeva; Anton Cherepov; Alexander Antipov, et al. The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals. Am. J. Life Sci. 2014, 3(1-2), 5-12. doi: 10.11648/j.ajls.s.2015030102.12

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    AMA Style

    Irina Alchinova, Elena Arkhipova, Yulia Medvedeva, Anton Cherepov, Alexander Antipov, et al. The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals. Am J Life Sci. 2014;3(1-2):5-12. doi: 10.11648/j.ajls.s.2015030102.12

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  • @article{10.11648/j.ajls.s.2015030102.12,
      author = {Irina Alchinova and Elena Arkhipova and Yulia Medvedeva and Anton Cherepov and Alexander Antipov and Nikolai Lysenko and Leonid Noskin and Mikhail Karganov},
      title = {The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {1-2},
      pages = {5-12},
      doi = {10.11648/j.ajls.s.2015030102.12},
      url = {https://doi.org/10.11648/j.ajls.s.2015030102.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030102.12},
      abstract = {Exposure to ionizing radiation leads to exhaustion of the pool of stem cells, increases differentiated cell stress, and activated reparation and apoptosis processes. Apart from well-documented acute effects, radiotherapy leads to delayed effects manifesting in few years after successful treatment. The aim of our study was the development of a test battery for evaluation of irradiation aftereffects in organisms characterized by different radiosensitivity. We chose C57BL mice as the ideal strain, because according to published data, they are characterized by medium radiosensitivity. At the preliminary stage, we have to assess informative value of some methods for the sublethal irradiation dose of 750 R. For evaluation of the damaging effects of radiation (lymphocyte count and maturity), we used, along with classical criteria, some complex parameters (body weight, serum homeostasis, behavior) characterizing general status of the organism. We also performed histological analysis of the liver, spleen, and pancreas and ranged the injuries by their severity.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals
    AU  - Irina Alchinova
    AU  - Elena Arkhipova
    AU  - Yulia Medvedeva
    AU  - Anton Cherepov
    AU  - Alexander Antipov
    AU  - Nikolai Lysenko
    AU  - Leonid Noskin
    AU  - Mikhail Karganov
    Y1  - 2014/11/14
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.s.2015030102.12
    DO  - 10.11648/j.ajls.s.2015030102.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 5
    EP  - 12
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2015030102.12
    AB  - Exposure to ionizing radiation leads to exhaustion of the pool of stem cells, increases differentiated cell stress, and activated reparation and apoptosis processes. Apart from well-documented acute effects, radiotherapy leads to delayed effects manifesting in few years after successful treatment. The aim of our study was the development of a test battery for evaluation of irradiation aftereffects in organisms characterized by different radiosensitivity. We chose C57BL mice as the ideal strain, because according to published data, they are characterized by medium radiosensitivity. At the preliminary stage, we have to assess informative value of some methods for the sublethal irradiation dose of 750 R. For evaluation of the damaging effects of radiation (lymphocyte count and maturity), we used, along with classical criteria, some complex parameters (body weight, serum homeostasis, behavior) characterizing general status of the organism. We also performed histological analysis of the liver, spleen, and pancreas and ranged the injuries by their severity.
    VL  - 3
    IS  - 1-2
    ER  - 

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Author Information
  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Dept. of Experimental Biology and Cell Physiology, Research Institute for Space Medicine, Federal Biomedical Agency of Russia, Moscow, Russia

  • Veterinary Dept., K. I. Skryabin Moscow state Academy of veterinary medicine and biotechnology, Moscow, Russia

  • Biology Dept., K. I. Skryabin Moscow state Academy of veterinary medicine and biotechnology, Moscow, Russia

  • Rectorate, Moscow state pedagogical University, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

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