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Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production

Received: 10 December 2014     Accepted: 13 December 2014     Published: 27 December 2014
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Abstract

Interferon-γ (IFN-γ) is a pleiotropic cytokine with antiproliferative and immunomodulatory activities that are crucial for the regulation of immune responses. We examined a group of military pilots. The examinees were divided into 3 subgroups: ground personnel (9 persons, control group), 17 pilots with <1000 h flight time, and 12 pilots with >1000 h flight time. No differences in IFN-α serum content after induction by NDV virus were detected. The quality of reparation is in many respects genetically determined; therefore, we used peripheral blood lymphocytes from pilots for in vitro detection of a radioadaptive response (RAR), which was evaluated by the number of chromosome aberrations. The adaptive response was observed in 7 individuals of the control group (78%), in 10 pilots who had <1000 flight hours (59%), and in 4 pilots having >1000 flight hours (33%). The examined individuals were divided into 2 groups depending on the presence of RAR, and IFN-γ production after radiation was measured. It was shown that at doses 0.05 Gy or 0.5 Gy no differences between groups were detected. Exposure with these doses sequentially in 48 h interval resulted to differently directed changes: lymphocytes of individuals with RAR produced more IFN-γ than before while cells of persons without RAR made it less. The quality of adaptive mechanists evaluated by RAR may be useful for estimation of individual sensitivity to radiation during radiotherapy in oncology and in prediction of professional risk.

Published in American Journal of Life Sciences (Volume 3, Issue 1-2)

This article belongs to the Special Issue Space Flight Factors: From Cell to Body

DOI 10.11648/j.ajls.s.2015030102.17
Page(s) 43-47
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Radioadaptive Response, Flight Factors, Interferon-γ, Individual Sensitivity

References
[1] Cavallo D., Ursini C.L., Carelli G., Iavicoli I. et. al., “Occupational exposure in airport personnel: Characterization and evaluation of genotoxic and oxidative effects,” Toxicology, Apr., 2006, pp. 16 – 18.
[2] Radiation Exposure and High-Altitude Flight, NCRP Commentary, 12, 1995.
[3] Bochkov N.P., Popova N.A., Katosova L.D. and al., “Extraordinarily high level of chromosomal variability in culture human peripheral blood lymphocytes,”Genetika, vol. 35, No. 6, 1999, pp. 838-841. (in Russian)
[4] Antoschina M.M., Fesenco E.V., Nasonova V.A., Ryabchenco N.I., “Adaptive response after preliminary irradiations of human lymphocytes,” 27th Annual Meeting of the European Society for Radiation Biology, vol. 32,1997, pp.407-408.
[5] Mortazavi S.M.J., Mozdarani H. “The search for a possible optimum adapting dose under the optimum irradiation time scheme in cultured human lymphocytes,”International Journal of Low Radiation, vol. 3, No. 1,2006, pp.74-82.
[6] B.S. Fedorenko, Iu.I. Voronkov, G.P. Snigireva, V.A. Shevchenko, S.V. Druzhinin, Iu.A. Akatov, V.V. Tsetlin, “Effect of space flight factors on health of cosmonauts in the near and late term after space flights,”Radiats Biol Radioecol, Nov-Dec, vol. 42, No. 6, 2002, pp.765-8. (in Russian)
[7] Nenoi M, Wang B, Vares G., “In vivo radioadaptive response: A review of studies relevant to radiation-induced cancer risk,” Hum Exp Toxicol., 2014 Jun 12, pii: 0960327114537537. [Epub ahead of print]
[8] David J.T.Jr., J.R. Patterson, C.Velasco-Gonzalez, E.N. Carroll, J. Trinh, D. Edwards, A.Aiyar, B. Finkel-Jimenez, and A.H. Zea, “Interferon-Gamma-Induced Nitric Oxide Inhibits the Proliferation of Murine Renal Cell Carcinoma Cells,” International Journal of Biological Sciences, vol. 8, No. 8,2012, pp. 1109-1120. doi: 10.7150/ijbs.4694
[9] Kaffman A., O’Shea E.K., “Regulation of nuclear localization: a key to a door,” Annu. Rev. Cell Dev. Biol, vol.15, 1999, pp.291-339.
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[11] Gupta P., Su Z., Lebedeva I.V. et al., “mda-7/IL-24: Multifunctional cancer-specific apoptosis-inducing cytokine,” Pharmacol. Therap., vol.11, No. 3, 2006, pp.596-628.
[12] Kawabe S., Nishikawa T., Munshi A., “Adenovirus-mediated mda-7 gene expression radiosensitizes non-small lung cancer cells via TP53-independent mechanisms,” Molec. Ther., vol. 6, 2002, pp.637-644.
[13] Yacoub A., Mitchell C., Lister A. et al., “Melanoma differentiation-associated 7 (interleukin 24) inhibits growth and enhances radiosensitivity of glioma cells in vitro and in vivo,” Clin. CancerRes., vol.9, 2003, pp.3272 – 3281.
[14] Makedonov GP, Tskhovrebova LV, Vasil'eva IM, Zasukhina GD.,“Radioadaptive response and antimutagenic effect of interferon have common pathways of cell protection against gamma radiation,”Dokl Akad Nauk, vol. 359, No. 6, 1998, pp. 838-40.(in Russian)
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  • APA Style

    Elena Arkhipova, Irina Alchinova, Mikhail Karganov. (2014). Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production. American Journal of Life Sciences, 3(1-2), 43-47. https://doi.org/10.11648/j.ajls.s.2015030102.17

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    ACS Style

    Elena Arkhipova; Irina Alchinova; Mikhail Karganov. Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production. Am. J. Life Sci. 2014, 3(1-2), 43-47. doi: 10.11648/j.ajls.s.2015030102.17

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    AMA Style

    Elena Arkhipova, Irina Alchinova, Mikhail Karganov. Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production. Am J Life Sci. 2014;3(1-2):43-47. doi: 10.11648/j.ajls.s.2015030102.17

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  • @article{10.11648/j.ajls.s.2015030102.17,
      author = {Elena Arkhipova and Irina Alchinova and Mikhail Karganov},
      title = {Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {1-2},
      pages = {43-47},
      doi = {10.11648/j.ajls.s.2015030102.17},
      url = {https://doi.org/10.11648/j.ajls.s.2015030102.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030102.17},
      abstract = {Interferon-γ (IFN-γ) is a pleiotropic cytokine with antiproliferative and immunomodulatory activities that are crucial for the regulation of immune responses. We examined a group of military pilots. The examinees were divided into 3 subgroups: ground personnel (9 persons, control group), 17 pilots with 1000 h flight time. No differences in IFN-α serum content after induction by NDV virus were detected.  The quality of reparation is in many respects genetically determined; therefore, we used peripheral blood lymphocytes from pilots for in vitro detection of a radioadaptive response (RAR), which was evaluated by the number of chromosome aberrations. The adaptive response was observed in 7 individuals of the control group (78%), in 10 pilots who had 1000 flight hours (33%). The examined individuals were divided into 2 groups depending on the presence of RAR, and IFN-γ production after radiation was measured. It was shown that at doses 0.05 Gy or 0.5 Gy no differences between groups were detected. Exposure with these doses sequentially in 48 h interval resulted to differently directed changes: lymphocytes of individuals with RAR produced more IFN-γ than before while cells of persons without RAR made it less. The quality of adaptive mechanists evaluated by RAR may be useful for estimation of individual sensitivity to radiation during radiotherapy in oncology and in prediction of professional risk.},
     year = {2014}
    }
    

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    T1  - Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production
    AU  - Elena Arkhipova
    AU  - Irina Alchinova
    AU  - Mikhail Karganov
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    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.s.2015030102.17
    DO  - 10.11648/j.ajls.s.2015030102.17
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 43
    EP  - 47
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2015030102.17
    AB  - Interferon-γ (IFN-γ) is a pleiotropic cytokine with antiproliferative and immunomodulatory activities that are crucial for the regulation of immune responses. We examined a group of military pilots. The examinees were divided into 3 subgroups: ground personnel (9 persons, control group), 17 pilots with 1000 h flight time. No differences in IFN-α serum content after induction by NDV virus were detected.  The quality of reparation is in many respects genetically determined; therefore, we used peripheral blood lymphocytes from pilots for in vitro detection of a radioadaptive response (RAR), which was evaluated by the number of chromosome aberrations. The adaptive response was observed in 7 individuals of the control group (78%), in 10 pilots who had 1000 flight hours (33%). The examined individuals were divided into 2 groups depending on the presence of RAR, and IFN-γ production after radiation was measured. It was shown that at doses 0.05 Gy or 0.5 Gy no differences between groups were detected. Exposure with these doses sequentially in 48 h interval resulted to differently directed changes: lymphocytes of individuals with RAR produced more IFN-γ than before while cells of persons without RAR made it less. The quality of adaptive mechanists evaluated by RAR may be useful for estimation of individual sensitivity to radiation during radiotherapy in oncology and in prediction of professional risk.
    VL  - 3
    IS  - 1-2
    ER  - 

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Author Information
  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

  • Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia

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